The simple, precise and accurate first derivative spectroscopic method has been developed for simultaneous estimation of gatifloxacin and flurbiprofen in bulk and eye drops. The solution of both the drugs and eye drops were prepared in methanol. Quantitative determination of the drugs was performed using double beam UV-visible spectrophotometer at 248 nm and 262 nm for gatifloxacin and flurbiprofen respectively. The method was linear in the concentration range of 20-70 μg/ml for gatifloxacin and 2-7 μg/ml for flurbiprofen with correlation coefficients of 0.9994 for both the drugs. The percentage recoveries obtained for gatifloxacin and flurbiprofen ranges from 98.40-101.25% and 98.5-101.60%, respectively. The method was validated and successfully employed for simultaneous estimation of both drugs in commercial products.
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