A rapid, precise, specific, accurate and robust simple RP-HPLC method for the simultaneous determination of rabeprazole sodium and domperidone has been developed and validated. The separation was carried out by using a mobile phase consisting of modified phosphate buffer pH 7.4 and acetonitrile in the ratio of 65:35% v/v. The column used was thermoscientific hypersil BDS, C18 (250 mm x 4.6 mm i.d., 5 μm) with flow rate of 1.5 ml/min using UV detection at 290 nm. The retention times of rabeprazole sodium and domperidone were found to be 3.8 min and 7.1 min, respectively. Method shows linearity in the range of 10 μg/ml-30 μg/ml for rabeprazole sodium and 5 μg/ml-15 μg/ml for domperidone with correlation coefficient of 0.9999 for both. The limit of detection (LOD) and limit of quantification (LOQ) for rabeprazole sodium and domperidone were found to be 0.08 μg/ml and 0.24 μg/ml and 0.06 μg/ml and 0.18 μg/ml respectively. The % recovery for rabeprazole sodium and domperidone were found within the range of 98.18% - 99.75% and 98.16% - 99.88% respectively. For both rabeprazole sodium and domperidone, the intra-day and inter-day precision, expressed as the relative standard deviation (RSD), of replicates is <2%. The developed RP-HPLC method was innovation, suitable for detecting both Rabeprazole sodium and Domperidone in soft gelatin capsules.
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