Without resolving mixtures of formoterol fumarate dihydrate and beclomethasone dipropionate, by application of various UV methods for simultaneous estimation of combined pharmaceutical formulation were developed as follows: in UV spectroscopic methods, estimation of formoterol fumarate dihydrate and beclomethasone dipropionate were carried out using methanol as solvent at max 214 nm and 239 nm for simultaneous equation method, 284 nm and 239 nm for absorption correction method, absorbance difference of 229 nm – 247 nm (difference is zero for FFD) and 208 nm – 218.40 nm (difference is zero for BD) for dual wavelength method and 267.20 nm (ZCP of FFD) and 306 nm (ZCP of BD) for first order derivative spectroscopic method respectively for FFD and BD. The methods obey beer’s law in concentration ranges employed for the estimation. The developed methods were validated in terms of linearity, LOD, LOQ, accuracy, precision and robustness as per ICH Q2 (R1) guidelines. Comparison of all developed and validated methods were carried out by using statistical tools. The method is successfully applied to pharmaceutical formulation, with no interference from excipients. Developed method was simple, accurate for routine simultaneous estimation of formoterol fumarate dihydrate and beclomethasone dipropionate.
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