A specific, accurate and simple RP-HPLC method for the simultaneous determination of Paracetamol and Ibuprofen has been developed and validated. The separation was carried out by using a mobile phase consisting of Acetonitrile:Water:Triethylamine (pH 3.5 adjusted by using Ortho phosphoric acid) in ratio of 60:40:0.1 (v/v/v). The column used was Princetons, C8 (250 mm x 4.6 mm i.d., 5 μm) with flow rate of 1 ml/min using UV detection at 220 nm. The retention times of Paracetamol and Ibuprofen were found to be 2.747 min and 10.428 min, respectively. Method shows linearity in the range of 6.5 μg/ml - 32.5 μg/ml for Paracetamol and 8 μg/ml - 40 μg/ml for Ibuprofen with correlation coefficient 0.9997 and 0.9995, respectively. The limit of quantification (LOQ) for Paracetamol and Ibuprofen were found to be 0.6992 μg/ml and 1.5218 μg/ml, respectively.
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