Novel combination of flurbiprofen sodium (FLB) and gatifloxacin (GATI) is available as eye drops form in the ratio of 1:10 and no spectrophotometric method has been reported yet. The present research work aims to develop a simple, sensitive, accurate and reproducible method for the simultaneous estimation of both drugs by the first order derivative spectroscopy, using methanol as a solvent. The method was performed at 247.60 nm (zero crossing point of FLB) and 262 nm (zero crossing point of GATI) for GATI and FLB sodium respectively. The regression analysis data for the calibration plot showed good linear relationship in the concentration range of 0.5-2.5 μg/ml (R2 = 0.9996) for FLB and 5-25 μg/ml (R2 = 0.9988) for GATI. The average percentage recovery of FLB and GATI combination was found to be 100.05% and 99.74% respectively. The LODs for FLB and GATI were 0.1057μg/ml and 0.4408 μg/ml and LOQs were found to be 0.3204 μg/ml and 1.3359 μg/ml respectively. Statistical analysis proves that the method is reproducible and selective for the simultaneous determination of FLB and GATI. The Results were found to be within acceptance criteria according to ICH Q2 R1 guidelines.
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