A simple, efficient and reproducible RP-HPLC method for the simultaneous determination of cilnidipine and metoprolol succinate in pharmaceutical dosage form has been developed and validated. The separation was carried out on hyperchrom ODS-BP (4.6 mm X 200 mm, 5 μm) column using acetonitrile: 0.05 M potassium dihydrogen phosphate buffer (adjusted to pH 3 with 10% OPA) in the ratio of 50:50 v/v as eluent. The flow rate was 1 ml/min and effluent was detected at 280 nm. The retention time of cilnidipine and metoprolol succinate was 3.36 and 6.25 min. respectively. The linear dynamic range was 2-10 μg/ml for cilnidipine and 10-50 μg/ml metorolol succinate, respectively. Percentage recoveries for cilnidipine and metoprolol succinate were 99.95 – 100.05% and 99.29 – 99.76% respectively. All the analytical validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed method is also found to be precise and robust for the simultaneous determination of cilnidipine and metoprolol in tablet dosage forms.
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