Two chemometric methods, Classical least square and Inverse least square were applied for simultaneous estimation of gatifloxacin and ketorolac tromethamine in their ophthalmic dosage form. A considerable spectral overlap (90.3%) was observed in the range of 281 nm-329 nm. Beer’s law was obeyed for both drugs in the concentration ranges of 6-18 µg/ml for both gatifloxacin and ketorolac tromethamine. 25 mixtures were prepared and analysed at 25 wavelength points between 281-329 nm with the interval of 2 nm (Δλ = 2 nm). A validation set design of the concentration data corresponding to the gatifloxacin and ketorolac tromethamine mixtures was organized statistically to maximize the information content from the spectra and to minimize the error of multivariate calibrations. Good percentage recoveries and proper statistical data obtained proved the suitability and efficiency of the proposed procedures for routine analysis. The numerical calculations were performed with the MATLAB® R2010a software and Microsoft excel.
Loading....