A simple, sensitive, precise and economical UV- spectrophotometric method has been developed for the simultaneous estimation of Naphazoline HCl and Phenylephrine HCl in formulation. Method is first order derivative spectrophotometric method and involved in the estimation of Naphazoline HCl and Phenylephrine HCl in water as solvent using the first-order derivative zero crossing point technique at 245 nm as ZCP of PHE and 283 nm as ZCP of NAPH. Calibration curve was prepared by plotting the derivative absorbance versus concentration. Linearity was obtained in the concentration range of 5-25 mcg/ml for NAPH and 12-60 mcg/ml for PHE. This method was successfully applied to pharmaceutical formulation because no interferences from formulation excipients were found. The suitability of this method for the quantitative determination of both drugs was proved by validation. The proposed method was found to be useful for the routine quality control application in eye drops.
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