Aim of the present work was to develop a stability indicating high performance liquid chromatographic (HPLC) method for the analysis of alfuzosin HCl and dutasteride. Chromatographic separation achieved isocratically on kromasil C18 column (150 mm x 4.6 mm, 5 μm particle size) utilizing a mobile phase of acetonitrile: phosphate buffer pH 7.0 in the ratio of 65:35 v/v at a flow rate of 1 ml/min and injection volume of 20 µl using UV detection at 242 nm. The retention time of ALFU and DUTA was 2.67, 9.97 min respectively. Both ALFU and DUTA were degraded in acidic, alkali and oxidation condition while only ALFU degraded in photolytic, DUTA in neutral condition. Both the drugs are stable in thermal condition. The method was found linear over concentration range of 20-140 μg/ml of ALFU and 1-7 μg/ml of DUTA with good linearity response of 0.999 and 0.998 respectively. The developed method was validated as per ICH guide line.
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