A simple RP-HPLC method has been developed and validated for simultaneous estimation of cefoperazone sodium and tazobactam sodium in parenteral preparation. The separation was carried out by a mobile phase consisting of phosphate buffer pH 4.0 adjusted with orthophosphoric acid and methanol (70:30). The column used was ODS hypersil C18, (5 μm, 250 mm × 4.6 mm) with flow rate of 1 ml / min using PDA detection at 223 nm. The described method was linear over a concentration range of 24-72 μg/ml and 3-9 μg/ml for the assay of cefoperazone sodium and tazobactam sodium respectively. The retention times of cefoperazone sodium and tazobactam sodium were found to be 3.3 and 5.1 min respectively. Results of analysis were validated statistically and by recovery studies. The limit of quantification (LOQ) for cefoperazone sodium and tazobactam sodium were found to be 5.38 and 0.84 μg/ml respectively. The results of the study showed that this RP-HPLC method is simple, rapid, precise and accurate, which is useful for the routine determination of cefoperazone sodium and tazobactam sodium bulk drug and in its pharmaceutical dosage form.
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