A simple, selective, rapid, precise and economical reverse phase high-pressure liquid chromatographic method has been developed for the simultaneous estimation of isosorbide dinitrate and hydralazine hydrochloride from pharmaceutical formulation. The method was carried out on a hypersil C18 (250 x 4.6 mm, 5 μm) column, with a mobile phase consisting of acetonitrile: phosphate buffer pH 3.6 (30:70 v/v) at a flow rate of 0.8 ml/min. The retention time of hydralazine hydrochloride and isosorbide dinitrate was 3.59, 6.56 min respectively. The developed method was validated in terms of accuracy, precision, linearity, limit of detection, limit of quantitation. The proposed method can be used for estimation of these drugs in combined dosage form for routine analysis.
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