Two methods for simultaneous estimation of camylofin dihydrochloride (CFD) and paracetamol (PCM) in combined tablet dosage form have been developed after derivatizing with potassium permanganate as a solvent. KMnO4 used as a derivatizing agent for increasing absorbtivity of CFD in UV spectroscopic methods. The first method is based on first order derivative method and second method is based on dual wavelength method. For the method I, the estimation of CFD was carried out at max 273 nm (ZCP of PCM) and of PCM at 374.5 nm (ZCP of CFD). For method II, estimation of CFD was carried out at max 259.5 nm and 382 nm (difference is zero for PCM) and of PCM at 254 nm and 270 nm (difference is zero for CFD). The methods obey beer’s law in concentration ranges employed for the estimation. The developed methods were validated in terms of linearity, LOD, LOQ, accuracy, precision and robustness as per ICH Q2 (R1) guidelines. The proposed methods have been applied successfully for the analysis of the drug in pure and in its pharmaceutical dosage forms. The results obtained from both methods were statistically compared using paired t test.
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