A RP-HPLC method was developed for the simultaneous estimation of metoprolol succinate and chlorthalidone in their combined dosage form. The separation was achieved by BDS hypersil C18 (250 x 4.6 mm) 5 μm column and phosphate buffer (pH 3.5 using O-phosphoric acid): methanol: acetonitrile (50:25:25 v/v/v) as mobile phase, at a flow rate of 1.0 ml/min having injection volume 20 µl. Detection was carried out at 223 nm. Retention time of metoprolol succinate and chlorthalidone was found to be 6.073 min and 3.500 min respectively. The method has been validated for linearity, accuracy and precision. Linearity for metoprolol succinate and chlorthalidone were in the range of 20-60 μg/ml and 6-14 µg/ml respectively. The % recoveries obtained for metoprolol succinate and chlorthalidone were found to be in range of, 99.93-100.02% and 100.18-101.57% respectively. The Stability Indicating RP-HPLC method were found to be accurate, precise, selective and stability indicating. Metoprolol succinate and chlorthalidone showed degradation in acidic, basic, oxidative, photolytic and thermal in appropriate condition.
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