An isocratic reversed phase HPLC method has been developed for the simultaneous determination of hydrocortisone acetate and miconazole nitrate on a HiQ-silC18HS (250 x 4.6 mm) column using a mobile phase consisting of acetonitrile: 0.1 M ammonium acetate (80:20, v/v) at a flow rate of 1 ml/min and the detection was carried out at 225 nm. The retention times of Hydrocortisone Acetate and Miconazole Nitrate were 4.055 (±0.16) min and 13.14 (±0.31) min respectively. The method was validated with respect to specificity, linearity, accuracy, precision, ruggedness and robustness. This method is simple, precise and sensitive and is applicable for simultaneous quantification of hydrocortisone acetate and miconazole nitrate in a cream formulation.
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