UV spectrophotometric method has been developed for simultaneous estimation of dyphylline (DYP) and guaifenesin (GUA) in bulk drug and in their combined dosage form by first order derivative. This method utilizes distilled water as a solvent and λmax of dyphylline and guaifenesin selected for analysis was found to be 273 nm (at ZCP of GUA) and 260 nm (at ZCP of DYP) respectively. Linearity was observed in the concentration range of 2-10 μg/ml for DYP (r2 = 0.998) and 20-100 μg/ml for GUA (r2 = 0.996). The accuracy and precision were determined and found to comply with ICH guidelines. This method showed good reproducibility and recovery with % RSD in the desired range. The proposed methods can be successfully applied for the routine analysis of both the drugs. This method was simple, rapid, accurate and sensitive.
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