A simple, rapid, precise, sensitive and reproducible high performance liquid chromatographic method was developed for estimation of minoxidil in tablet dosage form. Chromatographic separation of the drug was achieved on a phenomenex kinetex 5µ C-18 100 A column (250 × 4.6 mm) using a mobile phase, methanol and water (60:40% v/v) at a flow rate of 1 ml/min. The drug eluted was monitored at 235 nm. The retention time was 3.3 min. The calibration curve was linear over the range of 10 ng - 200 μg/ml. The performance of the method was validated according to ICH guidelines. The method can be applied for determination of drug in its tablet dosage form without any interference from excipients or degradant substances and also applicable to topical solutions (MINTOP). The proposed method is suitable for routine quality control analysis.
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