A simple, sensitive, precise, accurate, economic and rapid spectrophotometric method has been developed and validated for estimation of levonorgestrel using UV spectrophotometer. Maximum absorbance was observed at 241 nm using methanol as a solvent. Linearity was found in the concentration range of 2-20 μg/ml for levonorgestrel. The method was validated for linearity, precision, accuracy, limit of detection and limit of quantification as per ICH guidelines. The %RSD values were <2%. The average % recovery was found to be 99.91±0.26% for proposed method. The assay result obtained was 102.53±0.35% for levonorgestrel using proposed method. A stability indicating UV spectrophotometric method was developed for analysis of the drug in various degradation conditions. It involved acidic, alkaline, oxidative, thermal, UV and sunlight degradation conditions in which methanol were used as solvent. The amount of degraded drug was calculated by taking absorbance at 241 nm. The proposed method was found to be simple, economic and sensitive, hence can be used for the routine analysis and quality control checking of levonorgestrel in pharmaceutical dosage form.
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