The aim of this study was to develop and validate a dissolution test for the quality control of Atenolol and Indapamide, using RP-HPLC method. The optimised dissolution conditions includes USP apparatus II at a paddle rotation rate of 50 rpm and 900 ml of modified acetate buffer (pH=4.6) at 37°C±0.5°C. Under these conditions, the in-vitro release profiles of Atenolol and Indapamide showed good results. The drug release was evaluated by RP-HPLC using column Hyperchrom ODS 5 µ C18 (250X4.6 mm, 100°A), detection wavelength 240 nm having the flow rate 1.0mL/min using using methanol: water in the proportion of 80:20 and adjusted pH to 3.0 with10% orthophosphoric acid. The method validation was carried out as per for USP guidelines and it was found that the results obtained by proposed method for dissolution test for tablet formulation containing Atenolol and Indapamide are reliable, precise and accurate. Hence it was routinely adopted for dissolution analysis of the said drugs in the formulation.
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