A fixed dose combination of Ceftazidime (CEF-β lactam antibiotic) and Tazobactam (TAZO-β Lactamase inhibitor) is used in ratio of 8:1 as powder form of injection for the treatment of resistant lower respiratory tract and other infections. An attempt was done to develop simple precise, accurate and validated stability indicating HPTLC method for the estimation of CEF and TAZO in bulk and injectable dosage form. The compounds were separated on aluminium-backed silica gel 60 F254 plates with n-propanol: Water: Triethylamine (7.5:2.0:0.5 v/v/v) as mobile phase. These systems were found to give sharp spots of CEF and TAZO with the Rf value of 0.37 and 0.67 respectively. Densitometric analysis of CEF and TAZO were done at 254 nm. Regression analysis for the calibration plots was indicative of good linearity between response and concentration over the range 1000-6000 ng/band for CEF and 2000-12000 ng/band for TAZO with the correlation coefficient (r2) 0.9993 and 0.9991 respectively. CEF and TAZO were subjected to acid, Alkali, oxidative, dry heat and photo degradation. It was susceptible to acidic, alkaline and oxidative hydrolysis conditions. Statistical analysis proved that method is repeatable, selective, and accurate for simultaneous estimation of CEF and TAZO. So the method effectively separated the drugs from their degradation products, it can be used as a stability-indicating assay method.
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