The present manuscript describe simple, sensitive, rapid, accurate, precise and cost effective dual wavelength spectrophotometric method for the simultaneous determination of Amitryptiline HCl and Chlordiazepoxide in combined tablet dosage form. The utility of dual wavelength data processing program is its ability to calculate unknown concentration of components of interest in a mixture containing an interfering component. The principle for dual wavelength method is “the absorbance difference between two points on the mixture spectra is directly proportional to the concentration of the component of interest”. The method was based on determination of Amitryptiline HCl at the absorbance difference between 237 nm and 254 nm and Chlordizepoxide at the absorbance difference between 231 nm and 245 nm. 0.1 N HCl was taken as a solvent. Regression analysis of Beer’s plots showed good correlation range of 6-16 µg/ml for Amitryptiline HCl and 3-10 µg/ml for Chlordizepoxide with correlation coefficient r2 0.9976 and 0.9946, respectively. The mean % recoveries were found to be in the range of 98.63 % - 100.16 % and 98.89 % - 99.72 % for Amitryptiline HCl and Chlordizepoxide, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of pharmaceutical dosage form.
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