The present work describes a validated Reverse-phase High Performance Liquid Chromatographic method for simultaneous estimation of Cefpodoxime Proxetil and Ofloxacin in tablet dosage form. The separations were achieved using Inertsil C18 column, 5 µm, {250 mm × 4.6 mm (i.d.)} with a mobile phase consisting of 0.05 M KH2PO4 buffer (pH 3.0 adjusted with 0.1% orthophosphoric acid): Methanol: Acetonitrile (40:40:20 v/v/v), at the flow rate of 1.0 mL/min. Detection was carried at 235 nm using a variable wavelength UV-2070/2075 Plus detector. The retention time for Ofloxacin was about 3.167 minute and for Cefpodoxime Proxetil I and Cefpodoxime Proxetil II were about 12.933 and 14.451 minute respectively. The linearity range for Cefpodoxime Proxetil and Ofloxacin was 6.5-32.5 g/ml and 5-25 µg/ml, respectively. The proposed method was found to be accurate, precise, specific, and rugged and thus can be used for simultaneous estimation of Cefpodoxime Proxetil and Ofloxacin in tablet formulation.
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