A simple, precise and sensitive UV method has been develop for the estimation of Isoniazid (INH) in bulk drug form by Difference Spectrophotometric method. Isoniazid has exhibited maximum absorbance at about 266 nm and 270 nm in acidic and basic solution respectively. Beer's law was obeyed in the concentration range of (10-50) mcg/ml in both the cases. The proposed methods were successfully applied for the determination of Isoniazid in bulk drug. As per ICH guidelines the results of the analysis were validated statistically and were found to be satisfactory.
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