A simple, sensitive, accurate, precise and rapid reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Ambroxol hydrochloride, Levocetirizine dihydrochloride and Phenylephrine hydrochloride in combined dosage form. The chromatographic separation was performed on ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consisted of a mixture of phosphate buffer pH 6, acetonitrile and methanol in the ratio of 25: 10: 65, v/v/v at a flow rate of 0.8 ml/min. The detection wavelength was set at 230 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the concentration range of 2-30 μg/ml for Ambroxol hydrochloride, 2-30 μg/ml for Levocetirizine dihydrochloride and 2-30 μg/ml for Phenylephrine hydrochloride. The retention times were found to be 5.39±0.1 min for Ambroxol hydrochloride, 3.36±0.12 for Levocetirizine dihydrochloride and 2.49±0.13 for Phenylephrine hydrochloride. The mean recoveries were 100.06±1.52, 99.80±1.26 and 100.54±1.23 for Ambroxol hydrochloride, Levocetirizine dihydrochloride and Phenylephrine hydrochloride, respectively. The method can be easily adopted for quality control analysis.
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