A simple, efficient, and reproducible RP-HPLC method for the simultaneous determination of Cefixime trihydrat and Moxifloxacin HCl in pharmaceutical formulations has been developed and validated. The separation was carried out on Hyperchrom ODS-BP (4.6 mm X 200 mm, 5 μm) column using acetonitrile: 0.05 M potassium dihydrogenortho phosphate (adjusted to pH 4 with 10% OPA) in the ratio of 50:50 v/v as eluent. The flow rate was 1 ml/min and effluent was detected at 279 nm. The retention time of Cefixime and Moxifloxacin were 4.937 and 3.963 min. respectively. The linear dynamic range was 25-75 μg/ml for Cefixime and Moxifloxacin, respectively. Percentage recoveries for Cefixime and Moxifloxacin were 99.85-100.16% and 99.68-100.50% respectively. All the anal validation parameters were determined and found in the limit as per ICH guidelines, which indicates the validity of the method. The developed method is also found to be precise and robust for the simultaneous determination of Cefixime and Moxifloxacin in tablet dosage forms.
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