A simple, sensitive, accurate, precise and rapid reverse phase high performance liquid chromatographic method has been developed and validated for the simultaneous determination of Eperisone hydrochloride and Paracetamol from synthetic mixture. The chromatographic separation was performed on ACE 5 C18 column (150 mm × 4.6 mm i.d, 5 μm particle size). Mobile phase consisted of a mixture of phosphate buffer pH 6, acetonitrile and methanol in the ratio of 10: 30: 60, v/v/v at a flow rate of 0.7 ml/min. The detection wavelength was set at 255 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. The calibration was linear over the concentration range of 2-50 μg/ml for Eperisone hydrochloride and 2-50 μg/ml for Paracetamol. The retention times were found to be 2.2±0.14 min for Diclofenac sodium and 3.9±0.13 min for Eperisone hydrochloride. The mean recoveries were 99.2±0.59 and 98.5±0.43 for Eperisone hydrochloride and Paracetamol, respectively. The method can be easily adopted for quality control analysis.
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