A simple and economic UV spectrophotometric method for the simultaneous determination of betamethasone valerate and neomycin sulphate in pharmaceutical dosage form was developed. Betamethasone is a glucocorticoid. It is widely used in pharmaceutical preparation. Neomycin is an aminoglycosidic antibiotic, widely used against Gram-negative bacteria. Because of its advantages (simplicity, low cost and accessible instruments), Ultraviolet spectrophotometry represents a suitable method for the quantitative determination of pharmaceutical active ingredients in raw materials and marketed formulation. All readings were performed at two wavelengths 235 nm and 274 nm for betamethasone valerate and neomycin sulphate respectively. Accuracy was determined by spiking standards and the recoveries of betamethasone valerate and neomycin sulphate were found to be between 99.16-100.8% and 99.70-100.86% respectively. The method was validated and proved to be linear. The developed method is also the first report on the simultaneous determination of betamethasone valerate and neomycin sulphate in pharmaceutical preparation.
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