A simple, accurate and precise Q-absorbance Ratio UV spectrophotometric method for the simultaneous estimation of Ambroxol Hydrochloride and Doxofylline in Bulk and Pharmaceutical Dosage forms has been developed and validated. The method is based on Q- Absorption Ratio method using two wavelengths, 243.5 nm (λmax of Ambroxol hydrochloride) and 255 nm (iso-absorptive point). Distilled water was used as a solvent in the method. The method was validated with respect to linearity, accuracy, precision and robustness as per the International Conference on Harmonisation (ICH) guidelines. The drug response with respect to absorbance was linear over the concentration range 1-50 µg/ml for both Ambroxol hydrochloride and Doxofylline. The percentage recovery of Ambroxol Hydrochloride and Doxofylline was found to be 99.75% and 100.11% respectively. The %R.S.D. values for intra-day and inter-day precision study were <2.0%, confirming that the method was sufficiently precise. The method can be successfully employed for the simultaneous determination of Ambroxol hydrochloride and Doxofylline in pharmaceutical formulations.
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