Rifaximin is a structural analog of rifampin and a non-systemic, gastrointestinal site-specific antibiotic. Three simple, sensitive, precise and economical UV Spectrophotometric methods have been developed and validated in tablet formulation. Method A involved dissolving Rifaximin in chloroform and obtained an absorption maxima at 297 nm, Method B is based on first order derivative spectra which showed a sharp peak at 289 nm and Method C involved calculation of Area under Curve (AUC), that gives absorption in the wavelength range of 270-300 nm. Linearity for the detector response was observed in the concentration range of 2-20 µg/ml for method A and B, and 4-20 µg/ml for method C respectively. Results of the analysis were validated for accuracy, precision, Limit of detection (LOD), Limit of Quantitation (LOQ) and, found to be satisfactory. The proposed methods are simple, sensitivity, rapid, economical and suitable for the routine quality control application in pharmaceutical formulations.
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