A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Paracetamol (PCM) and Tapentadol (TP) by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 249 nm (zero cross point of Paracetamol) was used for quantification of tapentadol and 274 nm (zero cross point of tapentadol) for quantification of Paracetamol. The linearity was established over the concentration range of 4-12 µg/ml and 30-70 µg/ml for Paracetamol and Tapentadol with correlation coefficient r2 0.9993 and 0.9992, respectively. The mean % recoveries were found to be in the range of 98.85% – 99.56% and 98.70% – 99.69% for Paracetamol and Tapentadol, respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of pharmaceutical dosage form.
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