A new, precise, simple, and accurate spectrophotometric method was developed for the Simultaneous estimation of Moxifloxacin HCl and Loteprednol Etabonate in combined dosage form. The combination is effective in Prophylaxis of Post Operative steroidal intra ocular infection where risk of bacterial population high. The simultaneous estimation of Moxifloxacin HCl and Loteprednol Etabonate involve absorbance measure at 228 nm (zero-crossover point of Moxifloxacin HCl) and at 243.20 nm (zero-crossover point of Loteprednol Etabonate) in first order Derivative mode. The formulation was evaluated for the percentage of content of both drugs at selected wavelength. Both the drug obeys Beer’s Lambert’s Law in the range of 5-30 µg/ml. the method was successfully applied to the formulation without any interference from incipient. The developed method was validated as per ICH guideline.
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