A new, simple, rapid, accurate, sensitive and precise Q-Absorption Ratio Spectrophotometric method was developed and validated for the simultaneous estimation of Vardenafil and Dapoxetine Hydrochloride in combined tablet dosage form. The measurement of absorbance was performed at 276.0 nm (iso-absorptive point) and 292.0 nm (Absorption maxima of Dapoxetine Hydrochloride). Beer's law was obeyed over a concentration range of 7.5-30 μg/ml for Vardenafil (r2=0.9992) and 15-60 μg/ml for Dapoxetine Hydrochloride (r2=1.0000). The relative standard deviation was found to be <2.0%. The LOD for Vardenafil and Dapoxetine HCl were found to be 0.53 μg/ml and 0.675 μg/ml respectively. LOQ for Vardenafil and Dapoxetine HCl were found to be 1.59 μg/ml and 2.05 μg/ml respectively. The method showed good recovery for both the drugs indicating its accuracy. The method was validated according to ICH guidelines. A marketed tablet formulation was analysed by the developed method. The developed Q-ratio method can be successfully applied for routine analysis of Vardenafil and Dapoxetine Hydrochloride in bulk and combined dosage form without any interference of the excipients.
Loading....