This paper presents a RP-HPLC method for the simultaneous estimation of Cefixime and Linezolid in combined tablet dosage form. The process was carried out on C18 column (Luna 5 μm, 250 × 4.60 mm, i.d) using phosphate buffer pH 5.5 and methanol in the ratio of 50:50 v/v respectively as a mobile phase at a flow rate of 1.2 mL/min. Detection wavelength was fixed at isobestic point 268 nm. Linearity was observed in the concentration range of 10-50 μg/ml for Cefixime (r2=0.9988) and 30-150 μg/ml for Linezolid (r2=0.9978). The retention time of Cefixime and Linezolid was found to be 2.671 and 6.987 minutes, respectively. The developed method is simple, precise, specific, robust, rapid, reproducible, and sensitive and it can be used for estimation of Cefixime and Linezolid in combined tablet dosage form.
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