A simple, precise, accurate and reproducible spectrophotometric method has been developed for simultaneous estimation of Cefpodoxime proxetil (CPD) and Levofloxacin hemihydrate (LVX) by employing first order derivative zero crossing method in methanol. The first order derivative absorption at 235 nm (zero crossing point of Cefpodoxime proxetil) was used for quantification of Levofloxacin hemihydrate and 265 nm (zero crossing point of Levofloxacin hemihydrate) for quantification of Cefpodoxime proxetil. The linearity was established over the concentration range of 2-10 µg/ml and 2.5-10.5 µg/ml for Cefpodoxime proxetil (r2= 0.9985) and Levofloxacin hemihydrates (r2=0.9994) respectively. The mean % recoveries were found to be in the range of 99.30 % – 100.73 % and 99.67 % – 100.57 % for Cefpodoxime proxetil and Levofloxacin hemihydrate respectively. The proposed method has been validated as per ICH guideline and successfully applied to the estimation of Cefpodoxime proxetil and Levofloxacin hemihydrate in bulk drug and pharmaceutical dosage form.
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