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EVALUATE SUN PROTECTIVE ACTIVITY OF ETHANOLIC EXTRACT OF FRESH APPLE PULP VS. MARKETED COSMETIC FORMULATION
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Hemant S Kandle, Sachin R Jadhav, Akshay A Khot, Ganesh H Wadkar
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Sunscreens are chemicals that provide protection against the adverse effects of solar and in particular UV radiation. Natural substances extracted from plants have been recently considered as potential sunscreen resources because of their ultraviolet ray absorption in the UV region and their antioxidant activity. Present study deals with evaluation the UV protective activities of ethanolic extracts of fresh fruits using diffuse transmittance method and calculating Sun Protection Factors (SPFs). The apple from the market was randomly collected and extracted with ethanol. Diifferent dilutions (100mg&200mg/ml) were prepared in ethanol. The absorbance of all the aliquots were recorded at different nm with 5 nm intervals from 250-350 nm.The invitro SPF values were determined at wavelength from 290-320 nm according to the method discussed by Mansur. Apple extract has sun protection activity as the concentration of extract increases from 100-200ug/ml. The apple extract have protective activity as compared to marketed formulation for both UVA and B ranges that was statistically analyzed. The marketed lotion civers broad ranges of UV absorbance while fresh extracts absorbs skin erythmal producing UV- B radiation suggesting satisfactory sun protective as compared to marketed formulation....
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COMPARATIVE ANALYSIS OF SPF OF CALENDULA OFFICINALIS FLOWER, JUGLANS REGIA SEEDS AND FICUS BENGALENSIS BARK BY UV-VIS SPECTROSCOPY
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Harshit Shah*, Deepinderjeet Singh Joshan, Shruti Rawal Mahajan
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Sun is constantly emits radiation but this radiation also contain UV rays which has destructive effect on skin. UV radiation produces premature skin aging which is characterised by wrinkle formation, thinning of skin, spider vein formation etc. Appearance of these symptoms make person to feel embarrassment in society. To avoid such kind of situation it is necessary to use treatment, which provides protective effect against this. Ability of any formulation to provide protective effect is decided by SPF. Higher the SPF higher will be protective effect. SPF is determined by a cost effective UV-Vis spectroscopic method. Synthetic agents do have side effects on application but if agents exist in nature are having minimum or no side effects, so it will be safe and beneficial if we use such agents. In this article SPF of Calendula officinalis flower, Juglans regia seeds, and Ficus bengalensis bark is calculated by UV-Vis spectroscopic method....
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FORMULATION AND EVALUATION OF A NOVEL HERBAL GEL OF GLYCYRHIZA GLABRA EXTRACT FOR SUNSCREEN ACTIVITY
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Manjeet Singh Rathore*, Pankaj Kumar, Mohammad Asif, Prashant Desai, Badri
Prakash Nagori, Gajendra Kamal Singh
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Ultraviolet B (UVB) rays are the major cause of sunburns. The present study was designed to prepare Glycyrhiza glabra sunscreen gel. Glycyrhiza glabra L is well-known drugs for its therapeutic values. The sunscreen activities of gel formulations could be related to the free radical scavenging properties of Glycyrhiza glabra extracts incorporated in it. Sunscreen activity of herbal gel preparation from Glycyrhiza glabra was measured using UV –visible spectrophotometry method in the range of 200-400nm. Result of gel formulation showed maximum absorbance at 280-400nm that proved these sunscreen activity....
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ANTITYROSINASE EFFECT OF MAJOR FRACTIONS OF TERMINALIA CHEBULA RETZ. FRUITS
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Mehdi Ansari Dugaheh, Fariba Sharififar, Nahid Ahmadi, Amir Assadi Khanuki, Amin
Purhemati, Mandana Moshrefi
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Excessive melanin biosynthesis leads to skin hyperpigmentation which causes undesirable skin appearance. Our previous studies have shown high tyrosinase inhibitory effect of Termilalia chebula fruits. In the present work, we aimed to separate anti tyrosinase fractions of T. chebula fruits. Total extract of T. chebula was prepared by maceration method with methanol 80% and evaporated in vacuum. Dried extract was partitioned successively with petroleum ether, chloroform, ethyl acetate and methanol. Further fractionation of active fraction (methanolic) was performed by liquid-liquid extraction (LLE) to give 18 fractions. Active fractions identified by bioautography and were subjected for colorimetry method in different concentrations using mushroom tyrosinase and L-tyrosine as substrate. The percentage of inhibition and IC50 values of each sample were determined. Fractions ?V and V separated in ethyl acetate-methanol by LLE method exhibited the greatest percentage of tyrosinase inhibition (80.52 ± 1.8% and 76.81 ± 2.1% respectively) in comparison to kojic acid (81.28 ± 2.2%). The least IC50 values were also due to the fractions of ?V and V (IC50 values of 3.8 and 4.1 µg/ml respectively). Our findings indicated that the fractions of ?V and V have high anti tyrosinase effect which is compareable with kojic acid. Concidering the solvent of these fractions (methanol- ethyl acetate), they belongs to phenolic compounds. Further studies for separation of active compound have being carried out....
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IS THERE TRULY NO BENEFIT WITH SUNSCREEN USE AND BASAL CELL CARCINOMA? A CRITICAL REVIEW OF THE LITERATURE AND THE APPLICATION OF NEW SUNSCREEN LABELING RULES TO REAL-WORLD SUNSCREEN PRACTICES
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Cameron Chesnut, Jenny Kim
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Basal cell carcinoma (BCC) is the most common human malignancy. Both epidemiological and direct evidence have established
ultraviolet (UV) exposure from the sun as the most important risk factor for BCC development. There has only been one
randomized and controlled study to examine sunscreen’s role in the prevention of BCC, and no significant protective benefit
was found. This study did not address four important concepts: sunscreen abuse, sunscreen misuse, sunscreen formulation,
and cumulative UV exposure. Thus, the results of this study are difficult to interpret and extrapolate with real-world sunscreen
practices....
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QUANTIFICATION OF SUNSCREEN ETHYLHEXYL TRIAZONE IN TOPICAL SKIN-CARE PRODUCTS BY NORMAL-PHASE TLC/DENSITOMETRY
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Anna W Sobanska, Jaroslaw Pyzowski
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Ethylhexyl triazone (ET) was separated from other sunscreens such as avobenzone, octocrylene, octyl methoxycinnamate, and
diethylamino hydroxybenzoyl hexyl benzoate and from parabens by normal-phase HPTLC on silica gel 60 as stationary phase.
Two mobile phases were particularly effective: (A) cyclohexane-diethyl ether 1 : 1 (v/v) and (B) cyclohexane-diethyl ether-acetone
15 : 1 : 2 (v/v/v) since apart from ET analysis they facilitated separation and quantification of other sunscreens present in the
formulations. Densitometric scanning was performed at 300 nm. Calibration curves for ET were nonlinear (second-degree
polynomials), with R > 0.998. For both mobile phases limits of detection (LOD) were 0.03 and limits of quantification (LOQ)
0.1 µg spot-1. Both methods were validated....
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