Journal Scope
Inventi Rapid/Impact: Biomedical Analysis is the peer reviewed journal of Pharmaceutical Sciences consist of original research and reviews on pharmaceutical and biomedical analysis including developments in analytical methodology, instrumentation, computation and interpretation involving academic, clinical and industrial analysis in pharmaceutical sciences. This journal also has the papers related to Papers dealing with the analytical aspects of traditional folk medicines and Bioanalytical papers (pharmacokinetic, bioequivalence, protein and DNA binding studies).
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BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF CARBAMAZEPINE IN HUMAN PLASMA USING HYPHENATED LIQUID CHROMATOGRAPHY - TANDEM MASS SPECTROSCOPY (LC-MS/MS)
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Girish M Taviyad, Tejas B Parmar, Chagganbhai N Patel
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A simple bioanalytical method was developed to estimate Carbamazepine from human plasma with due consideration of cost effective sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of carbamazepine and Carbamazepine D10 using as internal standard(IS) in K2EDTA human plasma. Both the analytes were extracted by protein precipitation technique. The chromatographic separation was performed by isocratic on Inert sustain C18 100*, 4.6 mm,5µ with a mobile phase of 0.1% glacial acetic acid solution in water: organic mixture (35:65) (v/v)[organic mixture-methanol:acetonitrile(80:20)] followed by detection using mass spectrometry. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated and the lower limit of quantification for Carbamazepine and Carbamazepine D10 was found to be 50 ngmL-1 and 50 ngmL-1 respectively. The mean recovery for Carbamazepine and Carbamazepine D10 ranged from 89.34 to 98.01% and also this method no significant matrix effect on analytes. The method was validated over the concentration range of 50 ng/mL to 8000 ng/mL for both Carbamazepine and Carbamazepine D10 in human plasma with a accuracy of within run and between run in the range of 80-120%. The within run and between run precision of Carbamazepine and Carbamazepine D10 were also within the range of 20% ( for LLOQ level) and 15%( for other than LLOQ) of %CV....
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BIOANALYTICAL METHOD DEVELOPMENT AND VALIDATION OF RAMIPRIL AND ITS METABOLITE RAMIPRILAT IN HUMAN PLASMA BY USING LCMS/ MS
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Badmanaban R, Nilesh N Prajapati, Chagganbhai N Patel
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A simple, rapid, sensitive and specific liquid chromatography-tandem mass spectrometry method was developed and validated for quantification of Ramipril and its metabolite Ramiprilat in K2 EDTA human plasma. Both the drugs were extracted by solid phase extraction.Enalpril was used as the internal standard (IS). The chromatographic separation was performed by binary gradient on HYPURITY C18, 3x50 mm,3µ with a mobile phase of 0.1% formic acid solution in water: organic mixture (10:90) (v/v)[organic mixture-methanol:acetonitrile(90:10)] followed by detection using mass spectrometry. The protonated analyte was quantitated in positive ionization by multiple reaction monitoring with a mass spectrometer. The method was validated and the lower limit of quantification for Ramipril and Ramiprilat was found to be 0.390 ngmL-1and 0.395 ngmL-1 respectively. The mean recovery for Ramipril and Ramiprilat ranged from 95.63 to 85.66% and also this method no significant matrix effect on analytes. The method was validated over the concentration range of 0.300 ng/mL to 40.000 ng/mL for both Ramipril and Ramiprilat in human plasma with a accuracy of within run and between run in the range of 80-120%. The within run and between run precision of Ramipril and Ramiprilat were also within the range of 20% ( for LLOQ level) and 15%( for other than LLOQ) of %CV....
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EFFECT OF CALCIUM SUPPLEMENT ON ORAL BIOAVAILABILITY OF GATIFLOXACIN IN ALBINO RATS
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Patel Bimal, Krishnaveni N, Jivani Nurrudin, Khodakiya Akruti, Khodakiya Moorti,
Parida Saswat
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Gatifloxacin is identified as important antibacterial agent of class Quinolone antibiotic, acting by inhibiting the bacterial enzymes DNA gyrase and topoisomerase IV. Clinical studies show that cationic preparations forms complexes with quinolone antibiotics and decrease their absorption to various extents. The objective of this study was to investigate the effect of calcium on oral absorption of gatifloxacin in rats to establish a preclinical food supplement – drug interaction model. Gatifloxacin (400 mg) and Calcium supplement (containing 250 mg calcium) used for the study. The peak plasma concentration i.e. Cmax after administration of gatifloxacin was 5.1667 µg/ml at 3.0 hr (Tmax). The Cmax and Tmax of gatifloxacin administered along with calcium supplement were 3.4119 µg/ml and 3.0 hr, respectively. The relative bioavailability of the gatifloxacin tablet administered along with calcium supplement was 66.04 % in comparison with gatifloxacin tablet which is assumed to 100 %. This reduction in gatifloxacin bioavailability suggests that calcium may forms complex with gatifloxacin leads to the decrease in bioavailability. This food supplement – drug interaction rat model may be useful in prediction of potential food – drug interactions in humans, and also can be utilized as a preclinical tool for interaction study....
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PHARMACOKINETICS OF DIBUTYL PHTHALATE (DBP) IN THE RAT DETERMINED BY UPLC-MS/MS
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Li-Wen Chang, Mei-Ling Hou, Tung-Hu Tsai
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Dibutyl phthalate (DBP) is commonly used to increase the exibility of plastics
in industrial products. However, several plasticizers have been illegally used as clouding
agents to increase dispersion of aqueous matrix in beverages. This study thus develops a
rapid and validated analytical method by ultra-performance liquid chromatography with
tandem mass spectrometry (UPLC-MS/MS) for the evaluation of pharmacokinetics of DBP
in free moving rats. The UPLC-MS/MS system equipped with positive electrospray
ionization (ESI) source in multiple reaction monitoring (MRM) mode was used to monitor
m/z 279.25148.93 transitions for DBP. The limit of quantication for DBP in rat plasma
and feces was 0.05 µg/mL and 0.125 µg/g, respectively. The pharmacokinetic results
demonstrate that DBP appeared to have a two-compartment model in the rats; the area
under concentration versus time (AUC) was 57.8 ± 5.93 min g/mL and the distribution
and elimination half-life (t1/2, and t1/2,) were 5.77 ± 1.14 and 217 ± 131 min, respectively,
after DBP administration (30 mg/kg, i.v.). About 0.18% of the administered dose was
recovered from the feces within 48 h. The pharmacokinetic behavior demonstrated that
DBP was quickly degraded within 2 h, suggesting a rapid metabolism low fecal cumulative
excretion in the rat....
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PHARMACOKINETICS AND TISSUE DISTRIBUTION STUDY OF PRAERUPTORIN D FROM RADIX PEUCEDANI IN RATS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY (HPLC)
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Taigang Liang, Wenyan Yue, Xue Du, Luhui Ren, Qingshan Li
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Praeruptorin D (PD), a major pyranocoumarin isolated from Radix Peucedani,
exhibited antitumor and anti-inflammatory activities. The aim of this study was to
investigate the pharmacokinetics and tissue distribution of PD in rats following intravenous
(i.v.) administration. The levels of PD in plasma and tissues were measured by a simple
and sensitive reversed-phase high-performance liquid chromatography (HPLC) method.
The biosamples were treated by liquid-liquid extraction (LLE) with methyl tert-butyl ether
(MTBE) and osthole was used as the internal standard (IS). The chromatographic
separation was accomplished on a reversed-phase C18 column using methanol-water
(75:25, v/v) as mobile phase at a flow rate of 0.8 mL/min and ultraviolet detection wave
length was set at 323 nm. The results demonstrate that this method has excellent specificity,
linearity, precision, accuracy and recovery. The pharmacokinetic study found that PD fitted
well into a two-compartment model with a fast distribution phase and a relative slow
elimination phase. Tissue distribution showed that the highest concentration was observed
in the lung, followed by heart, liver and kidney. Furthermore, PD can also be detected in the
brain, which indicated that PD could cross the blood-brain barrier after i.v. administration....
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PLASMA AND WHOLE BRAIN CHOLINESTERASE ACTIVITIES IN THREE WILD BIRD SPECIES IN MOSUL, IRAQ: IN VITRO INHIBITION BY INSECTICIDES
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Ashraf S Alias, Muna H I Al-Zubaidy, Yaareb J Mousa, Fouad K Mohammad
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Plasma and brain cholinesterase activities were determined in three wild bird species to assess their exposure to organophosphate
and carbamate insecticides which are used in agriculture and public health. In the present study, we used an electrometric method for
measurement of cholinesterase activities in the plasma and whole brain of three indigenous wild birds commonly found in northern
Iraq. The birds used were apparently healthy adults of both sexes (8 birds/species, comprising 3–5 from each sex) of quail (Coturnix
coturnix), collard dove (Streptopelia decaocto) and rock dove (Columba livia gaddi), which were captured in Mosul, Iraq. The mean
respective cholinesterase activities (? pH/30 minutes) in the plasma and whole brain of the birds were as follows: quail (0.96 and
0.29), collard dove (0.97and 0.82) and rock dove (1.44 and 1.42). We examined the potential susceptibility of the plasma or whole
brain cholinesterases to inhibition by selected insecticides. The technique of in vitro cholinesterase inhibition for 10 minutes by the
organophosphate insecticides dichlorvos, malathion and monocrotophos (0.5 and 1.0 µM) and the carbamate insecticide carbaryl
(5 and10 µM) in the enzyme reaction mixtures showed significant inhibition of plasma and whole brain cholinesterase activities to
various extents. The data further support and add to the reported cholinesterase activities determined electrometrically in wild birds
in northern Iraq. The plasma and whole brain cholinesterases of the birds are highly susceptible to inhibition by organophosphate
and carbamate insecticides as determined by the described electrometric method, and the results further suggest the usefulness of
the method in biomonitoring wild bird cholinesterases....
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E- ISSN: Awaited
Inventi Rapid Biomedical Analysis
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Frequency: Quarterly
E- ISSN: Awaited
RI Factor- 1.0
Abstracted/ Indexed in: Ulrich’s International Periodical Directory & Google Scholar, SCIRUS, Journal Seek (Genamics), PSOAR, getCITED
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