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Inventi Rapid: Pharm Analysis & Quality Assurance
publication date: 2013/3/12

STABILITY INDICATING HPLC ASSAY METHOD DEVELOPMENT FOR DETERMINATION OF ACEBROPHYLLINE
                  Ketkee S Jinnawar, Sneha Jawanjal, Krishna R Gupta*

ABSTRACT
A simple, precise and specific reverse phase high performance liquid chromatographic method has been developed and validated for the determination of acebrophylline in capsule. It was found that the excipient in the tablet dosage forms does not interfere in the quantification of active drug by proposed method. The HPLC separation was carried out by reverse phase chromatography on Shimadzu binary gradient system consisted of phenomenex ODS 5µ C18 column (250 X 4.6 mm), SPD – 10 UV detector and LC 10 ADVP Pumps. Rheodyne injector with 20 µl loop with a mobile phase composed of Ammonium Phosphate buffer + Methanol, pH 5 (70:30) at flow rate 1.0 mL/min. The detection was monitored at 273 nm. The calibration curve for acebrophylline was linear from 25-150µg/mL. The interday and intraday precision was found to be within limits. Accuracy (recoveries: 99.8-103.2%) and reproducibility were found to satisfactory. The method was found to be specific against excipient interference and stress condition. Stress testing showed degradation product and impurity were well-separated from the parent compound, conforming stability-indicating capacity of the method.

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How to Cite This Article

Ketkee S Jinnawar, Sneha Jawanjal, Krishna R Gupta*,  " STABILITY INDICATING HPLC ASSAY METHOD DEVELOPMENT FOR DETERMINATION OF ACEBROPHYLLINE ",  Inventi Rapid: Pharm Analysis & Quality Assurance ,  Vol. 2013 , Article ID- " Inventi:ppaqa/790/13 " 2013  [ cited 2014 Jul 28 ]  Available From  


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