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Inventi Rapid: Pharm Analysis & Quality Assurance
publication date: 2013/5/24

DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND CHLORZOXAZONE IN THEIR COMBINED TABLET DOSAGE FORM
                  Vinod Maheshwari, Jaydeep Sangani, Naimish Kariya, Payal Maheshwari, Jenisha Rokad

ABSTRACT
A simple, sensitive, precise, accurate and rapid RP-HPLC method has been developed and validated for the simultaneous determination of Diclofenac sodium and Chlorzoxazone in combine dosage form. The RP-HPLC method was performed on sunshell C18 column (100 mm × 4.6 mm i.d., 2.5 µm particle sizes). Mobile phase consisted of a mixture of phosphate buffer (0.05 M KH2PO4, pH adjusted to 3.2 using Orthophosphoric acid): Acetonitrile : TEA (55: 45: 1 v/v/v) at a flow rate of 1.5 ml/min. The detection wavelength was at 240 nm. The proposed method was validated for linearity, accuracy, precision, LOD and LOQ. Retention time of Diclofenac sodium and Chlorzoxazone was 4.94 ± 0.07 & 1.28 ± 0.03, respectively with linearity range of 20-60 µg/mL and 100-300 µg/mL for Diclofenac sodium and Chlorzoxazone respectively. The mean recoveries were 100.19 ± 0.518728 and 99.55 ± 0.536201 for Diclofenac sodium and Chlorzoxazone, respectively. The LOD value were found to be 0.3428 and 4.3289 µg /mL, while LOQ value were found to be 1.0387 and 13.1187 µg /mL for Diclofenac sodium and Chlorzoxazone respectively. This method can now transfer to utilize for routine laboratory analysis and assay of Diclofenac sodium and Chlorzoxazone in Their Tablet Dosage Form. Key-Words: Diclofenac sodium, Chlorzoxazone, RP-HPLC, Simultaneous method.

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How to Cite This Article

Vinod Maheshwari, Jaydeep Sangani, Naimish Kariya, Payal Maheshwari, Jenisha Rokad,  " DEVELOPMENT AND VALIDATION OF RP-HPLC METHOD FOR SIMULTANEOUS ESTIMATION OF DICLOFENAC SODIUM AND CHLORZOXAZONE IN THEIR COMBINED TABLET DOSAGE FORM ",  Inventi Rapid: Pharm Analysis & Quality Assurance ,  Vol. 2013 , Article ID- " Inventi:ppaqa/1049/13 " 2013  [ cited 2014 Jul 31 ]  Available From  


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