Inventi Rapid: Biomedical Analysis
publication date: 2012/6/6

METHOD DEVELOPMENT AND METHOD VALIDATION OF BROMOCRIPTINE USING LIQUID CHROMATOGRAPHY-MASS SPECTROMETRY IN HUMAN PLASMA
                  M Srinivasa Reddy*, I Mrutyunjaya Rao, N Someswara, M Anji Babu, N Sai Kiranmai

ABSTRACT
A new, rapid, simple and highly selective liquid chromatography/tandem mass spectrometry (LC/MS/MS) was developed and evaluated to determine the concentration of bromocriptine in human plasma. The method of extraction of drug is done by solid phase extraction using strata-X 33µ polymeric reverse phase cartridges. The drug and internal standard Quetiapine Hemifumarate, were separated on Gemini C18 100*46mm, 5µ column using mobile phase constituting 2mM Ammonium triflouroacetate: Acetonitrile (10:90v/v), which was analyzed using Turbo ESI mode. The developed method had a chromatographic run time of 5 min and a linear calibration curve over the concentration range of 10.016pg/ml to 509.711pg/ml with a regression value of 0.99. The lower limit of quantification was found to be 10.016pg/ml. Accuracy and Precision values for LLOQ QC are 112.8% and 10.4%. The method developed can be applied for the bioavailability and bioequivalence studies.

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